Forests are degraded from various factors, and the first step in restoration frequently involves revegetation. One of the degradations is wildfires, which damage vegetation, affect soils, and lead to the loss of ecosystem functions. Using seed mixtures is a viable method for restoring the ecosystems. This research investigated the impacts of six perennial plant seed mixtures derived from native plants in South Korea and the addition of two types of annual plant seeds to these mixtures, both separately and combined. Cultivation of the seed mixtures was conducted by pot cultivation in a greenhouse for the early stage of vegetation (16 weeks). The seed mixture treatment enhanced plant species diversity, number, and biomass. The seed mixture treatment elevated the urease activity from 14.42 to values between 33.88 and 55.74 μg NH₄-N g⁻¹ 2 h⁻¹. A seed mixture integrated with two annual plants heightened the phosphomonoesterase activity from 482.79 to 543.75 μg p-nitrophenol g⁻¹ h⁻¹. Nitrogen leaching was reduced across all seed mixture treatments, while phosphorus leaching diminished with the addition of the annual legume. These findings illustrate the influence of seed mixture treatments and the inclusion of annual seeds on the beginning stage of revegetation, offering a basis for further ecosystem restoration.