Prescribed fires are an important management tool for containing woody plant encroachment in rangeland ecosystems. Grasses are the dominant fuel type in rangelands. Past work has shown that grass canopy architecture, which varies among grass species, can influence flammability. Whether variation in grass fuel structure can influence postfire plant responses has not yet been tested. To bridge this gap, we set up field burning experiments with different fuel treatments and examined postfire mortality of Juniperus virginiana L. in a tallgrass prairie in southwestern Missouri. We sampled 60 trees and measured tree height and diameter at breast height before the fire. Fuels surrounding each tree were manipulated to vary independently in both fuel load and fuel structure. Flame temperatures were measured during the fire, and both stem and canopy injuries were evaluated 1 d after the fire. We surveyed tree mortality 7 mo after the fire. We found no effects of either fuel load or fuel structure on postfire mortality or on canopy injury in J. virginiana. Canopy injury was a critical fire severity measurement determining postfire mortality in J. virginiana, and taller trees are more fire resilient. Despite laboratory-observed fuel structure effects on flammability, this study finds no evidence for the importance of grass fuel load and canopy architecture in influencing postfire tree response. This result might arise from the low crown depth and low canopy water content of J. virginiana, which can promote canopy fire and result in a high mortality rate across fuel treatments. Notwithstanding the negative results, testing laboratory-based findings in field settings is important for further examining laboratory observations and upscaling individual-level processes to ecosystems to help identify the key ecological processes determining population dynamics and community assembly. Our study also suggests that prescribed fire is an effective tool to remove encroaching J. virginiana in tallgrass prairies at an early stage.